Number & Oligo & TM & Maximum crosshybridization TM \\
1 & 3'-$GGTGACCTCTGTCCCTTCCGA$-5' & 76.5757 & 41.722 \\
2 & 3'-$CCCTACCGCACTCCAGTCCC$-5' & 77.9118 & -273.15 \\
3 & 3'-$TCTCCCAGAGCCGTCCGGTCC$-5' & 82.5749 & -273.15 \\
4 & 3'-$AAGTCCGGGTCGGTCTGTCC$-5' & 76.9782 & 44.5121 \\
5 & 3'-$GGCCACGATGCCAATCCCGGC$-5' & 78.2308 & -273.15 \\
6 & 3'-$TAGGCCGCCACGTCACTTACC$-5' & 77.1609 & -273.15 \\
7 & 3'-$GCTCCCTTGCTACCTCTCTCC$-5' & 74.4813 & 29.9123 \\
8 & 3'-$CTCCCCGTTGACTTCCTTCGA$-5' & 74.0305 & 50.9857 \\
9 & 3'-$CCGTCCCCCAGCCTGCTCGT$-5' & 80.3238 & -273.15 \\
10 & 3'-$TGTCCCCGTACTGGTCACCTG$-5' & 75.9301 & 48.7479 \\
11 & 3'-$AGGGCGTTCTGTGTCGGTCCA$-5' & 76.9143 & 48.3398 \\
12 & 3'-$GACGTGGGACGCTCGGACGTA$-5' & 77.0059 & 46.5919 \\
13 & 3'-$GGGCTTGGGGCCCCCTGGGC$-5' & 87.9474 & -273.15 \\
14 & 3'-$TACCCGCGTGCCAGTCACT$-5' & 75.1727 & -273.15 \\
15 & 3'-$AGGAGGGTCAGGGCTCCCC$-5' & 79.1483 & -273.15 \\
16 & 3'-$TGACGGTTGCCACTTCGGTCC$-5' & 77.3868 & 33.5062 \\
17 & 3'-$CCCTCCGCATTGTCCCCGCCC$-5' & 83.0869 & 39.0845 \\
18 & 3'-$CCTGTCCCCGGACTCCTCC$-5' & 76.3503 & 34.1335 \\
19 & 3'-$TCCCACCCGGTCTCCCGCC$-5' & 82.5276 & 44.8389 \\
20 & 3'-$GTCGGGGCTGCCTTGGGCT$-5' & 78.9839 & -273.15 \\
21 & 3'-$TGTGGTCGTGGGGCCTTCTCA$-5' & 79.1106 & 52.2675 \\
22 & 3'-$CCCCTCACTTTCCCCCCACCC$-5' & 82.6154 & 40.5728 \\
23 & 3'-$TGAACCCCTCCACCCCGGAGA$-5' & 82.9275 & 42.5056 \\
24 & 3'-$TGTCCGGGCGTCCTTAGCT$-5' & 74.8222 & -273.15 \\
25 & 3'-$TCCCCTGAAGGGCGGACCC$-5' & 82.3907 & 37.8918 \\
26 & 3'-$GGGTAGTGGTCGCCCTTGGGC$-5' & 81.1796 & 52.689 \\
27 & 3'-$TGAACCTACTGGCCGTTGGGT$-5' & 76.048 & 39.6547 \\
28 & 3'-$AGGGCTTGGTCGCCCCTGG$-5' & 78.9587 & 45.2859 \\
29 & 3'-$CGGCCGCTGCTCGTTGAGGGC$-5' & 80.6938 & 38.8488 \\
30 & 3'-$GGAACAGGGCTTGGGTGCCCC$-5' & 81.1455 & 42.023 \\
31 & 3'-$TCGGAGCCCGAGCCTCTCC$-5' & 80.0771 & 24.4626 \\
32 & 3'-$TCCGCTCGACGAGGACGAGG$-5' & 77.1085 & 52.1299 \\
33 & 3'-$GGGGAGGGTGGCGTCCTTT$-5' & 77.3631 & -273.15 \\
34 & 3'-$CCTGACTCCCCCCCAACCC$-5' & 79.9748 & 40.307 \\
35 & 3'-$ATGCTCCCTGTTCTCCGTCC$-5' & 73.1761 & 46.6088 \\
36 & 3'-$TTACTTTGGTACCGCCCCACA$-5' & 73.7989 & 33.0429 \\
37 & 3'-$AGTAGTCTCCCCCCTTGGGCT$-5' & 78.0403 & 52.5059 \\
38 & 3'-$CGTGGTCTTCCCCCTGGTGTC$-5' & 78.1956 & 49.6197 \\
39 & 3'-$GGTTCCCATTGGAACCTCGGT$-5' & 72.7991 & 46.7538 \\
40 & 3'-$TCACGTCAACCACTGCGGACA$-5' & 72.9684 & 30.5415 \\
41 & 3'-$GACCCTGTGTCTCACACCGTC$-5' & 74.4514 & 43.327 \\
42 & 3'-$CGTGACGGTTGGTGTGTCCCA$-5' & 76.911 & 51.3694 \\
43 & 3'-$GCGGAGGGTCGGGTCTCCC$-5' & 78.9914 & 48.5384 \\
44 & 3'-$GCGGAGGGTTGGGTCTCCC$-5' & 76.5853 & 43.82 \\
45 & 3'-$ACGAGGTGGGCATCCTCCCCT$-5' & 79.4758 & 37.6806 \\
46 & 3'-$TGCCCTTGGGCCTTCCGGGC$-5' & 84.4496 & 40.5306 \\
47 & 3'-$TTCCTCCGCCACCGCTCGT$-5' & 77.4465 & 51.8493 \\
48 & 3'-$GGCCTCGGGAGTCGGGGCG$-5' & 82.3543 & 50.3281 \\
49 & 3'-$AGGAGGGTCCGGGCTCCCC$-5' & 82.4693 & 43.6566 \\
50 & 3'-$CGTGGGTGGACCCGTTCCT$-5' & 74.5884 & 34.89 \\
51 & 3'-$TCCCCCCACACCCGACCCGA$-5' & 85.2371 & 49.3932 \\
52 & 3'-$TGGGTGGTGGCCATCCCGGCA$-5' & 84.722 & 50.8554 \\
53 & 3'-$AGGGACAGGGTGTGGCCGTCC$-5' & 82.5216 & -273.15 \\
54 & 3'-$CCCTGGTGATGGGGGTCCCGT$-5' & 80.7483 & 47.1549 \\
55 & 3'-$GGCTGGGGGTGCACCACCCCC$-5' & 84.9722 & -273.15 \\
56 & 3'-$CCCCCCACCAAGTCCCGCCCT$-5' & 85.8539 & -273.15 \\
57 & 3'-$CCAACCGGAAGGGCGTCGCCC$-5' & 83.3792 & 52.5102 \\
58 & 3'-$TTCGCCCCCGCTGCGTCTGC$-5' & 82.0185 & 17.7851 \\
Conduct Experiment at 62.7991 degrees Celsius.