Please note that thermalign is deprecated and the newer program FractTerm should be used. Click here to use FractTerm

ThermAlign is a program to determine the melting temperature of two DNA sequences using a nearest-neighbor model.
The version used here requires two DNA-sequences as input. The program can be used with DNA-RNA and RNA-RNA duplexes if the respective thermodynamic parameters are provided. Unpaired bases (bulges) in the duplexes will be considered by the software.

Please insert your data in the following form.

Sequence 1
A sequence in 5' -> 3' direction
Sequence 2
A sequence in 3' -> 5' direction
Concentration of the probe Mol/L
Concentration of Na+ Mol/L
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